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TRIS BUFFER LR
TRIS BUFFER LR
Tris buffers are widely used for DNA agarose electrophoresis. The two main buffers are TBE (Tris borate/EDTA) and TAE (Tris acetate/EDTA). Although there are some differences in the resolution of different forms of DNA and their mobility during electrophoresis, these Tris buffers can generally be used interchangeably.
Tris, or tris(hydroxymethyl) aminomethane, is a common biological buffer, used throughout the DNA extraction process. During extraction from any number of sources, DNA is pH sensitive. During cell lysis, removal of unwanted cellular components and precipitation, tris is used to maintain a stable pH.
To acquire a buffering capacity it sould be in equilibrium with the corresponding acid which is the protonated form of Tris. Traditionally, protonation of Tris is accomplished by addition of HCl. pH of the solution decreases to the desired value and the resulting Tris-HCl solution is what we commonly call Tris buffer
Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) is a commonly used buffering solution. The role of Tris-HCl in molecular biology is to control the acidity and osmolarity of a solution. Due to its properties, Tris-HCl is often a component of lysis buffers.